Principle of operation

Principle of operation of the Unit for inactivation of RNA and DNA viruses in medical instruments

The Unit uses the principle of photoactivation of a methylene blue solution into which medical instruments are submerged. The main property of methylene blue is its high degree of tropism for RNA and DNA, that is, the ability to firmly bind to DNA and RNA due to the replacement in it of all molecules of a pair of nucleic acids – guanine and cytosine. The loss of all nucleic acids of guanine and cytosine by the RNA and DNA molecules of a virus completely deprives them of their ability to replicate — to obtain “prints” and to synthesize new RNA and DNA molecules in the cells of the microorganism.

In the Unit methylene blue solution is irradiated with monochromatic light flux with a wavelength of 590 nm. A special radiation source has been developed for this. Photoactivation of methylene blue is a result of matching of the absorption wavelength of methylene blue to the wavelength of monochromatic light flux. During photoactivation, the absorption of quantum energy by methylene blue molecules causes the excitation of electrons in their atoms. As a result, the molecules themselves become sources of scattered light of the same wavelength (classical scattering). Light quanta emitted by photoactivated methylene blue molecules cause photoactivation of molecules “in the shadow” relative to the monochromatic emitter of the “device”.

The second essential effect is that upon photoactivation of methylene blue latter contributes to the formation in solution of a large amount of atomic oxygen (O-), or so-called oxygen radicals. In the presence of oxygen radicals, processes accompanied by reduction of molecules intensify sharply, in particular, the process of nucleic acid cleavage from RNA and DNA molecules, in place of which the methylene blue molecule is firmly attached, which is the basis for inactivation of RNA and DNA viruses. The second essential effect is that upon photoactivation of methylene blue latter contributes to the formation in solution of a large amount of atomic oxygen (O-), or so-called oxygen radicals. In the presence of oxygen radicals, processes accompanied by reduction of molecules intensify sharply, in particular, the process of nucleic acid cleavage from RNA and DNA molecules, in place of which the methylene blue molecule is firmly attached, which is the basis for inactivation of RNA and DNA viruses. The second essential effect is that upon photoactivation of methylene blue latter contributes to the formation in solution of a large amount of atomic oxygen (O-), or so-called oxygen radicals. In the presence of oxygen radicals, processes accompanied by reduction of molecules intensify sharply, in particular, the process of nucleic acid cleavage from RNA and DNA molecules, in place of which the methylene blue molecule is firmly attached, which is the basis for inactivation of RNA and DNA viruses. The second essential effect is that upon photoactivation of methylene blue latter contributes to the formation in solution of a large amount of atomic oxygen (O-), or so-called oxygen radicals. In the presence of oxygen radicals, processes accompanied by reduction of molecules intensify sharply, in particular, the process of nucleic acid cleavage from RNA and DNA molecules, in place of which the methylene blue molecule is firmly attached, which is the basis for inactivation of RNA and DNA viruses.

The second essential effect is that during photoactivation of methylene blue, the latter promotes the formation in the solution of large amounts of atomic oxygen (O—), or the so-called oxygen radicals. The presence of oxygen radicals sharply intensifies the processes accompanied by a reduction of molecules. As a result, nucleic acids are ripped off from the RNA and DNA molecules and to which methylene blue molecules are firmly attached. This effect is the basis of inactivation of RNA and DNA viruses.  Oxygen radicals actively affect the molecules, which are exposed to direct monochromatic light flux, as well as the molecules located in the “shadow”.

This principle of operation of Unit for the inactivation of RNA and DNA viruses on medical instruments works at room temperature. Therefore, it eliminates the need for sterilization of medical instruments at high temperatures (autoclaving) or in harsh chemical solutions.

The Unit for inactivation of RNA and DNA viruses on medical instruments is intended for use in hospitals, clinics and medical offices and will have great demand.